At the end of a discussion on the Mullaperiyar and Cauvery disputes, DMK wanted to raise some points which the Speaker did not allow.
Agitated DMK members then trooped down to the Speaker's chair, inviting warnings from him to return to their seats.
But some of them squatted before the speaker's chair instead, prompting him to summon the Marshals.
Noisy scenes prevailed for a while even as the DMK members were removed physically.
Later, the House adopted a resolution moved by finance minister O Panneerselvam to suspend the DMK members for two days.
DMK president M Karunanidhi, who represents the Tiruvarur constituency in the assembly, was not present in the House.