The diary, posted on Soria Galvarro's website, was penned in blue ink from January to October 1967, a period when Guevara was on an ill-fated campaign to ignite a peasant revolution in Bolivia.
He was killed October 8, 1967 by Bolivian troops.
Guevara's diary was turned over to Cuba, which published it in 1968.
But the published texts contained changes from the original manuscript, sometimes to clean up Che's rawer language.
"Now everyone can see a virtual image of what he wrote in his handwriting," Soria Galvarro explained.
Soria Galvarro has written five books about Guevara and his time in Bolivia.