Suu Kyi spoke of her sadness that India, which had been one of her staunchest supporters, changed tack in the 1990s and began to engage with Myanmar's junta at a time when it was a pariah in the West.
"I have been asked whether I have been disappointed that India had not stood staunchly by us through the years of struggle for democracy," she said at an annual lecture in memory of India's first post-independence prime minister Jawaharlal Nehru.
"I was saddened to feel that we have drawn away from India or rather India has drawn away from us during our very difficult days," she added.
The Nobel peace prize winner, who was released from military house arrest in 2010, had earlier been praised for her "indomitable courage" at talks with Indian Prime Minister Manmohan Singh.
Singh had invited Suu Kyi to New Delhi when he visited Myanmar in May to try to boost trade and counter the influence of regional rival China.
India shares a 1,640 kilometre (1,020 mile) border with its northeastern neighbour Myanmar, and the two former British colonies have a long shared history.
Suu Kyi's father General Aung San -- regarded as Myanmar's independence hero -- was a personal friend of Nehru.