Mukhejee, who has been preparing the pitch of the stadium since 1985, shot a letter to Cricket Association of Bengal (CAB) on Saturday morning and sought a "medical leave". He also hinted that he may not re-join at all.
The development came less than 48 hours after the BCCI sidelined the 83-year-old and sent East Zone ground and pitch committee representative Ashish Bhowmick to prepare a customised wicket at the Eden.
"It was an insult for me," Mukherjee said referring to the appointment of Bhowmick.
Mukherjee is peeved at CAB's attitude and said President Jagmohan Dalmiya had even threatened to suspend him if he spoke about the pitch.
"Nowhere the president has the right to talk about pitches. But here they are threatening to suspend me if I talk about pitches. This is the treatment I get after more than two decades of my service at CAB," said Mukherjee, who is said to have differences with Indian skipper Mahendra Singh Dhoni.
Dhoni had demanded a rank turner for the Kolkata Test and Mukhejree had said that such a demand was not logical as two pitches cannot be the same. That had apparently prompted BCCI to send Bhowmik to oversee the preparations.
"I expected some sort of protection from CAB thought that they would stand by me. Instead they are after me. My BP shot up 170/100 last night. After the check-up, the doctors have advised me a complete rest for a month. I've sent the medical report and request for a medical leave for a month to the CAB this morning," he said after sending his application to CAB.
"I don't make pitches to earn money. I had prepared pitches in Bangladesh for the U-19 World Cup (in 2004) and the ICC Cup without getting a single penny. Cricket is my passion that's why I'm associated with Eden for so long."