The event was organised by Silicon Andhra, a cultural organisation run by US-based Telugus, at the GMC Balayogi stadium in Hyderabad.
A representative of the Guinness Book, who observed the event said it was the largest Kuchipudi dance.
The record participation of dancers is the result of painstaking efforts made by the organisers during the last few months.
Kuchipudi, the 600-year-old dance form, originated in Krishna district of Andhra Pradesh. Union minister D Purandeswari, who was present on the occasion, appreciated the efforts of the organisers and the dancers.