Fifth seeds Bhupathi and his Russian partner Nadia Petrova lost 3-6, 6-3, 11-13 to the Australian combine of Jarmila Gajdosova and Matthew Ebden.
Sania and Bryan frittered away seven of eight break points and were broken twice, losing the opening set in 37 minutes. The second set unfolded in similar fashion as Hradecka and Cermak closed the match after another 28 minutes.
In contrast, Bhupathi and Petrova were in for a more gruelling battle that lasted nearly one and a half hour, but ended up paying for five double faults in the decider to lose out narrowly.