Shane Watson hit 143 as Australia was bowled out for 298 by England in the fifth and deciding one-day international at The Rose Bowl on Monday.
Captain Michael Clarke added 75 in a 163-run partnership with Watson for the fourth wicket, leaving England with a tough target of 299 to clinch the series 2-1.
Ben Stokes took 5-61 and Chris Jordan claimed three wickets for England, which lost the toss.
Two of the first four ODIs in the series were abandoned because of rain. This the final match of Australia's tour of England. Australia have already lost the Ashes 3-0 earlier this summer.