Nine more people succumbed to influenza A (H1N1) virus on Wednesday, taking the total number of swine flu deaths in the country since Aug 3 to 75.
Five of the latest deaths took place in Maharashtra, the worst hit state, while Karnataka and Uttarakhand accounted for three and one deaths respectively, the health ministry said.
At least 177 people also tested positive for the contagious virus.
Though details of the deaths from Karnataka and Uttarakhand are not known, the dead in Maharashtra included a 28-year-old businessman in Mumbai and a 35-year-old woman in Pune, the city which has seen most fatalities.
Sandeep Gaikwad died of swine flu at the Hiranandani Hospital in Mumbai, said an official.
Gaikwad was hospitalised three weeks ago in serious condition. Despite the long treatment, the resident of Kandivli suburb in northwest Mumbai passed away early Wednesday.
Of the total deaths since the first death took place in Pune Aug 3, 41 people have died in Maharashtra, 19 in Karnataka, seven in Gujarat, three each in Delhi and Tamil Nadu and one each in Uttarakhand and Kerala.
At least 34 people, who have tested positive for swine flu, are being treated in hospitals, according to the Mumbai Disaster Control Room. In Pune, a 35-year-old woman died at the Sassoon Hospital.
Shabana Quadir Shaikh was admitted to the hospital four days ago, the State Swine Flu Control Room said.
There were also concerns about delayed test results in various places in the country.
The test results of a two-year-old girl in Bhopal, who was the first H1N1 patient in the city and who has already recovered, came only Tuesday evening -- 12 days after her samples were sent for verification.
Doctors at the State Swine Flu Control Room said the girl was admitted to the Gandhi Medical College Hospital Aug 13 with flu-like symptoms and her samples were sent for testing the same day.
"My child is now in good health," said VK Shrivastava, father of the girl, Ananya. "She was administered medicines for normal fever and cold, and has improved without medicines prescribed for swine flu."
According to the health ministry, 177 people tested positive for the virus on Wednesday, taking the total number of swine flu infections in India to 3,273.
Of the new infections, only one has travel history. The rest appear to have contacted the disease within the country.
According to the World Health Organisation (WHO), 182,166 confirmed cases have been reported worldwide till Aug 21 while 1,799 people have died, mostly in Mexico and the US.