It is all in the genes whether you indulge in cat-like clawing or pack the punches of a macho man, for a study of fruit flies has shown that a gene known as "fruitless" is a key factor underlying sexual differences in behaviour.
The findings mark a milestone in an unlikely new animal model for understanding the biology of aggression and how the nervous system gives rise to different behaviours.
"Aggression is a very serious problem in society, and it's a problem with a biological and genetic component," said co-author Edward Kravitz, the George Packer Berry professor of neurobiology at HMS, who developed the fruit fly fighting model used. "We want to understand that. I can't think of a better system to study than fruit flies. And no one gets hurt."
The fruitless gene is known for its role in male courtship. The large gene makes a set of male-specific proteins found exclusively in the nervous system of fruit flies, in about 2 per cent of neurons.
The proteins are necessary for normal courting. Males missing the proteins do not court females, and they sometimes court males, other research groups have shown. Females with a male version of the gene perform the male courting ritual with other females.
The same gene directs another sex-specific behavioGay or lesbianr – fighting patterns, the new study shows. Female fighting, for example, largely involves head butts and some shoving. Males prefer lunges; they rear up on their back legs and snap their forelegs down hard – sometimes nailing an opponent that is slow to retreat.
The flies undergo a major role reversal when the male and female gene versions are switched. With a feminine fruitless gene, male flies adopt more ladylike tactics, mostly the head butt and some shoving. With the masculine fruitless gene, females instinctively lunge to the exclusion of their usual maneouvres.
The gender-bending fruit flies were first developed to study courtship in the Austrian lab of co-author Barry Dickson, director of the Institute of Molecular Pathology. Dickson created male flies with the female version of the gene and female flies with the male version.
Meanwhile, co-author Steven Nilsen, a postdoctoral fellow in Kravitz's lab, had similar questions and was staging contests between another line of mutant fruitless flies without such clear brain-switching genetics. So Austrian postdoctoral fellow Eleftheria Vrontou, the lead author, packed up their flies and took them to the Boston fruit fly fight club.
"Ed has systematically developed reproducibly aggressive behaviour in flies and paved the way for serious analysis," said Laurie Tompkins, programme director at the National Institute of General Medical Sciences, which funds the work.
The fruit fly aggression model is part of a new trend to use fruit flies as models to study complex behaviour, including sleep and responses to painful stimuli, Tompkins said. "Drosophila have marvellous advantages in terms of genetic tricks," she said, "and flies in many respects behave and respond similarly to humans."
The findings provide a welcome guidepost to help enable future research to track down the underlying neural circuitry, said Bruce Baker, a biology professor at Stanford who first linked the fruitless gene to male-specific courtship behaviour.