John Lennon's Gretsch 6120 guitar is expected to fetch more than £400,000 pounds at an auction in central London.
The Gretsch 6120 model was played by Lennon when the band recorded Paperback Writer at London's famous Abbey Road studios in 1966, the BBC reported.
The guitar is being sold by cousin of the late Beatles' member, who was given the guitar in 1967.
The guitar was developed in the 1950s and was favored by musicians including blues legend Eric Clapton and Pete Townshend of The Who. The guitar has been described on auction website tracksauction.com as "one of the most significant of singer's guitars" to come on to the market in the last 30 years".
The recent auctions of other Beatles memorabilia have included the front door of a Liverpool home once lived in by Paul McCartney, and jackets worn by George Harrison and Ringo Starr.