Saina was leading 20-17 in the decider and was serving for the match when a line call, which looked clearly out, went in Hashimoto's favour, and the world number two and top seed Indian squarely laid the blame for here defeat to the controversial decision.
"I was really upset with line call and could not focus after that. It was quite bad as I failed to find my rhythm after that. I just could not control after that and it was totally my mistake," she said after losing her second round match.
"I was match point 17-20 up and I thought that the match was almost over but I had to play those points (after that line call) but I was totally not able to focus," a disappointed Saina said.
The London Olympics bronze medallist went down to Yui Hashimoto of Japan 21-13 12-21 20-22 in the women's second round at the Siri Fort Sports Complex in New Delhi.
"I tried my best today to pull off the match but that did not happen. The line call was not with me ... it's okay," said the top seeded shuttler.
World number two Saina said after the line call, she hardly got time to get back into momentum.
"Everything happened so fast. Rallies were happening so fast I did not have enough time to control and after the line call I just gave one point away at 20-19, which was a smash error. And it was all over," she explained.
Saina said that it was only in India Open that somehow she was unable to make a comeback.
"I don't really come back only in India Open. I generally get back in whatever situations but I don't know why I am not able to get back here," she said.
Last year also Saina, then world number five, lost in the round two against Korea's Youn Joo Bae.
The Hyderabad-based shuttlers' crashing out means that she would not be facing compatriot P V Sindhu in a much-anticipated quarterfinal clash. Had Saina won today, it would have been the first clash between the two in a tournament.
"Sindhu is doing well and I hope she continue to do well in this tournament," said Saina.
Talking about her Japanese opponent today, Saina said, "All Japanese are very good fighters and Hashimoto also did well."