Rising Canadian star Eugenie Bouchard saw her pre-Wimbledon preparations go awry after losing 2-6, 6-3, 6-3 to American Vania King in the first round of the WTA/ATP 's-Hertogenbosch Open on Tuesday.
Former Wimbledon junior champion Bouchard, who pulled out of last week's tournament in Birmingham after reaching the semi-finals of the French Open, will now arrive at next week's tournament at the All England Club with just one grass-court match under her belt this season.
13th-ranked Bouchard, who also made the last four of the Australian Open in January, raced through the opening set backed by a pair of service breaks.
But the 20-year-old was pegged back in the second set with King, the world number 75, capitalising on a lone break of serve.
Bouchard was forced to fend off a pair of break points at the start of the deciding set but the third seed came unstuck in her following service game as King made the vital breakthrough to take a 2-1 lead.
Bouchard had opportunities to pull herself back into the match as four break points came and went with the Canadian struggling to find her rhythm on the grass before King sealed the contest after just over two hours on court.
Second seed Dominika Cibulkova was also sent tumbling out in the Netherlands after losing 6-2, 3-6, 6-3 to Kazakhstan's Yaroslava Shvedova.
French Open quarter-finalist Garbine Muguruza swept aside Yvonne Meusburger of Austria 6-4, 6-1 in her second-round match, while fellow Spaniard Carla Suarez Navarro, who also reached the last eight at Roland Garros, eased past Barbora Zahlavova Strycova 6-4, 6-2 in round one.
Men's second seed Fernando Verdasco, the highest-ranked player in the field following the withdrawal of compatriot David Ferrer, recovered from a slow start to beat Frenchman Paul-Henri Mathieu 2-6, 6-4, 6-4 in the opening round.
Spanish third seed Roberto Bautista Agut also breezed into the last 16 with a routine 6-1, 6-3 win over Daniel Gimeno-Traver.
However, Halle finalist Alejandro Falla was unable to repeat last week's heroics as he was dumped out at the first hurdle by eighth seed Juergen Melzer of Austria.