Former Wimbledon champion and third seed Maria Sharapova was knocked out of the tournament in the second round by Portuguese qualifier Michelle Larcher De Brito on a dramatic Wednesday.
The tall Russian was scheduled on Court Two and never looked happy against a tenacious opponent ranked 131 in the world, losing 6-3 6-4 to leave the women's draw in tatters after the earlier withdrawal of second seed Victoria Azarenka.
Former world No.1 Sharapova needed treatment when trailing 4-3 in the second set after slipping and doing the splits behind the baseline, holding her hip after tumbling to the ground.
She went off court to be treated and resumed after a near 10-minute stoppage but the 2004 champion could not avoid one of her worst results at the All England Club.
Andy Murray emerged unscathed on a day of astonishing injury mayhem at Wimbledon as the world number two eased into the third round with a 6-3, 6-3, 7-5 win over Taiwan's Lu Yen-Hsun.
The US Open champion's opponent in the last 32 will be Spanish 32nd seed Tommy Robredo.
The famed grass courts were at the centre of a safety row on Wednesday after Azarenka, Steve Darcis, the conqueror of Rafael Nadal, and Jo-Wilfried Tsonga were among seven players forced out with injury.
World number two and Australian Open champion Azarenka withdrew just minutes before she was due on Centre Court to face Italian veteran Flavia Pennetta for a place in the third round.
A right knee injury, suffered in a fall during her first round win over Maria Joao Koehler on Monday which left her in tears and requiring 10 minutes of treatment, put paid to her hopes after an MRI scan showed extensive bruising to the knee.
French sixth seed Tsonga quit his match with Latvia's Ernests Gulbis with a knee injury at the end of the third set.
Also withdrawing were Darcis, who put out Nadal in the first round, Croatian 10th seed Marin Cilic and Kazakhstan's Yaroslava Shvedova.
John Isner and Radek Stepanek both quit during their second round matches.
The seven pullouts were the most on one day in Grand Slam history.
Azarenka's fall happened on Court One, the same arena where Darcis knocked out Nadal on Monday. On Centre Court, Tsonga needed a medical time-out to have his left knee taped just after he had dropped the second set, 6-3, against world number 39 Gulbis.
An All England Club spokesman said: "The courts have been prepared in exactly the same way as they have done in the past. There is no change. It is the same seed, everything is identical. We will definitely be looking at it. But we understand there is no connection that we're aware of with the preparation of the courts."