Uruguay and South Korea made one change apiece in their line-ups for Saturday's World Cup last 16 clash at the Nelson Mandela Bay stadium.
Uruguay coach Oscar Tabarez welcomed back Villarreal defender Diego Godin, with Mauricio Victorino dropping to the bench.
His South Korean counterpart, Huh Jung-Moo, called up Kim Jae-Sung in midfield in place of attacking midfielder Yeom Ki-Hun.
The two sides are meeting for only the second time on the World Cup stage, with Uruguay snatching a late goal to prevail 1-0 in a first round match in Italy in 1990.
Two-time former champions Uruguay made it to this stage of the competition as Group A winners after an opening goalless draw with France, a 3-0 win over hosts South Africa and a closing 1-0 defeat of Mexico.
South Korea, semi-finalists on home turf in 2002, have made it to the second round for the first time on foreign soil as Group B runners-up.
They opened with a 2-0 defeat of Euro 2004 champions Greece in Port Elizabeth, before a 4-1 wipe out by Argentina and secured their qualification with a 2-2 draw with Nigeria.
The winner of this match will go on to face either the United States or Ghana in the quarter-finals in Johannesburg on July 2.
Fernando Muslera; Maximiliano Perreira, Diego Lugano (capt.), Diego Godin, Jorge Fucile, Egidio Arevalo, Diego Perez, Alvaro Pereira, Diego Forlan, Luis Suarez, Edinson Cavani
Coach: Oscar Tabarez
Jung Sung-Ryong; Cha Du-Ri, Cho Yong-Hyung, Lee Jung-Soo, Lee Young-Pyo, Lee Chung-Yong, Ki Sung-Yueng, Kim Jung-Woo, Park Ji-Sung (capt), Kim Jae-Sung, Park Chu-Young
Coach: Huh Jung-Moo
Referee: Wolfgang Stark (GER)