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A Russian convoy of 280 trucks carrying humanitarian aid for Ukraine set off on Tuesday amid Western warnings against using help as a pretext for an invasion.
With Ukraine reporting Russia has massed 45,000 troops on its border, Nato said there was a "high probability" that Moscow could intervene militarily in the country's east, where Kiev's forces are closing in on pro-Russian separatists.
Western countries believe that Russian President Vladimir Putin - who has whipped up the passions of Russians with a nationalist campaign in state-controlled media since annexing Crimea from Ukraine in March - could now send his forces into the east to head off a rebel defeat.
Itar Tass news agency said the convoy has departed from near Moscow which means it would take it a couple of days to arrive in east Ukraine, some 1,000 km (620 miles) to the southwest.
"It has all been agreed with Ukraine," Business FM radio quoted President Vladimir Putin's spokesman, Dmitry Peskov, as saying.
Thousands of people are believed to be short of water, electricity and medical aid due to the fighting. US President Barack Obama said that any Russian intervention without Kiev's consent would be unacceptable and violate international law.
European commission president Jose Manuel Barroso also warned on Monday "against any unilateral military actions in Ukraine, under any pretext, including humanitarian".
Russia said aid would be delivered together with the International Committee of the Red Cross (ICRC).
The ICRC said on Monday it had submitted a document to Russian and Ukrainian officials. However, the independent agency said that it needed agreement from all parties as well as security guarantees to carry out the operation, as it does not use armed escorts.
"The practical details of this operation need to be clarified before this initiative can move forward," said Laurent Corbaz, head of ICRC operations for Europe and Central Asia.
According to UN agencies, more than 1,100 people have been killed including government forces, rebels and civilians in the four months since the separatists seized territory in the east and Kiev launched its crackdown.