Two-time champion Serena Williams lost at Wimbledon one round shy of another showdown with sister Venus.
Still rusty following a layoff and slowed by an ankle injury, Serena was upset by fellow American Jill Craybas 6-3, 7-6 (4).
Williams departed hours after her older sister advanced to the fourth round by beating Daniela Hantuchova 7-5, 6-3.
Venus will next play Craybas, who arrived at Wimbledon with a career record of 7-23 in Grand Slam tournaments.
The match was scheduled as the last of the day on Centre Court, but because other matches there ran long, Williams and Craybas were moved to Court 2 - the "Graveyard of Champions".
Past losers there have included Andre Agassi, Jimmy Connors, John McEnroe and seven-time champion Pete Sampras in his final match at Wimbledon.
Williams made a late decision to play at Wimbledon following a six-week layoff because of a left ankle injury. Earlier this week she said for the first time that her ankle was slightly broken.
On a chilly, cloudy afternoon, other women's winners included Lindsay Davenport, Kim Clijsters and Maria Sharapova. None lost a set.
{{/usCountry}}On a chilly, cloudy afternoon, other women's winners included Lindsay Davenport, Kim Clijsters and Maria Sharapova. None lost a set.
{{/usCountry}}Two-time defending champion Roger Federer and 2004 runner-up Andy Roddick advanced in men's play.
Williams earned her seventh Grand Slam title in January at the Australian Open but hasn't won a tournament since, and it was clear from the start this week she wasn't at her best.
She was pushed to three sets in the opening two rounds for the first time at a Grand Slam event, and Saturday's match was just her fourth in 2 months.