Structure of key player in antibiotic resistance: Study

The team used cryo-electron microscopy (cryo-EM) -- a process that freezes proteins and uses beams of electrons to obtain high-resolution microscopic images -- to elucidate the structure of T4SS.

With antibiotic-resistant bacteria on the rise, scientists have been searching for ways to shut down the Type IV secretion system (T4SS), a protein complex on the outer envelope of bacterial cells that helps them to exchange DNA with neighbouring bacteria and resist antibiotics.

Now a collaboration between UT Southwestern computational biologist Qian Cong, PhD, and molecular biologists at the University of London has elucidated the structure of the T4SS complex, providing a blueprint that could help researchers design drugs that slow the development of antibiotic resistance. Their findings were published in Nature.

"For the first time, we determined the 3D structure of the entire T4SS complex," said Dr. Cong, Assistant Professor of Biophysics and in the Eugene McDermott Center for Human Growth and Development at UTSW.

The team in London was led by Gabriel Waksman, Ph.D., whose lab has been working for more than two decades to understand T4SS, especially how it forms a thin, hollow structure called a pilus, which connects to nearby bacteria to share genes. For this project, his team used cryo-electron microscopy (cryo-EM) -- a process that freezes proteins and uses beams of electrons to obtain high-resolution microscopic images -- to elucidate the structure of T4SS. This was no small feat since the T4SS complex is larger than 99.6% of all those included to date in the worldwide library of protein structures.

"In addition to the contribution we have made toward the development of drugs to slow the spread of antibiotic resistance genes, this study showcases the power of modern computational methods to validate experimental results and suggest functional insights beyond available experimental data," said Dr. Cong, a Southwestern Medical Foundation Scholar in Biomedical Research.

This story has been published from a wire agency feed without modifications to the text. Only the headline has been changed.