Thriller named Most Groundbreaking
The 14-minute zombie epic, directed by London filmmaker John Landis, topped a poll for MySpace UK.
Second in the list was Here It Goes Again by US act OK Go in which the band members perform a dance routine on treadmills.
Britney Spears' breakthrough hit, ...Baby One More Time - which saw the then teenage singer dancing in school uniform - finished in third position.
A-Ha's animated promo for Take On Me, propelling the Norwegian trio to worldwide stardom - was fourth.
In fifth place was Johnny Cash's poignant black and white film for Hurt - shot while he was in poor health just six months before he died from diabetes complications.
Will Francis, site editor of MySpace UK, said modern videomakers could learn a lesson from the past.
"Only two of the top ten were made this century. Which shows despite massive advances in technology, a brilliant idea coupled with a great star is an unbeatable format," Sky News quoted him as saying.
The complete top ten:
1. Thriller - Michael Jackson (1983)
2. Here It Goes Again - OK Go (2006)
3. ... Baby One More Time - Britney Spears (1998)
4. Take On Me - A-Ha (1985)
5. Hurt - Johnny Cash - (2003)
6. Bohemian Rhapsody - Queen (1975)
7. Daft Punk - Around the World (1997)
8. Weapon Of Choice - Fatboy Slim (1999)
9. Sledgehammer - Peter Gabriel (1986)
10. Sabotage - Beastie Boys (1994)